自然科学版 英文版
自然科学版 英文版
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中南大学学报(英文版)

Journal of Central South University

Vol. 22    No. 8    August 2015

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Enzymatic reaction of ethanol and oleic acid by lipase and lignin peroxidase in rhamnolipid (RL) reversed micelles
Bao Shan(包珊)1, 2, Yuan Xing-zhong(袁兴中)1, 2, Peng Xin(彭馨)1, 2, Zeng Guang-ming(曾光明)1, 2, Wu Hai-peng(武海鹏)1, 2, Wang Hou(王侯)1, 2, Liu Huan(刘欢)1, 2, Ma Yue-jie(马玉洁)1, 2, Cui Kai-long(崔凯龙)1, 2, Wu Xiu-lian(吴秀莲)1, 2

1. College of Environmental Science and Engineering, Hunan University, Changsha 410082, China;
2. Key Laboratory of Environmental Biology and Pollution Control of Ministry of Education (Hunan University), Changsha 410082, China

Abstract:an environment friendly bio-surfactant of rhamnolipid (RL) was used as a solvent. The enzymatic reaction of oleic acid catalyzed by lipase and lignin peroxidase (lip) was evaluated. The optimum conditions of enzymatic reaction catalyzed by lipase (lip) were water to amphiphile molar ratio of 30 (20), RL of 60 (60) critical micelle concentration (CMC), pH of 7.0 (3.0) and temperature of 40 (30) °C, respectively. The change of enzyme conformation indicates that, for catalytic of lipase, water content is the most important factor of the enzymatic reaction of oleic acid, and pH for lip. With individual optimum conditions, the enzymatic efficiency of oleic acid catalyzed by lipase is higher than that by lip. In the presence of ethanol, the enzymatic reaction of oleic acid catalyzed by lipase suits Ping-Pong Bi-Bi mechanism. As an alternative to chemical reversed micelles, the RL reversed micelles are promising methods to enzymatic reaction of oleic acid.

 

Key words: lipase; lip; enzymatic reaction; rhamnolipid; reversed micelles; oleic acid

中南大学学报(自然科学版)
  ISSN 1672-7207
CN 43-1426/N
ZDXZAC
中南大学学报(英文版)
  ISSN 2095-2899
CN 43-1516/TB
JCSTFT
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