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中南大学学报(自然科学版)

Journal of Central South University

第46卷    第12期    总第256期    2015年12月

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文章编号:1672-7207(2015)12-4719-06
利用PCR-DGGE分析茂名油页岩矿区土壤细菌群落组成
蒋绍妍1,王文星2,薛向欣1,徐坤2

(1. 东北大学材料与冶金学院,辽宁沈阳,110819;
2. 东北大学生命科学与健康学院,辽宁沈阳,110819
)

摘 要: 为了了解油页岩矿区土壤环境中细菌群落的物种组成结构,以野外多点采集广东茂名油页岩矿区土壤样品作为研究对象,采用改进的SDS高盐提取法提取微生物总DNA,并利用PCR-DGGE技术分析细菌群落菌属种类。结果表明:DGGE指纹图谱中共包含13个操作分类单元(OTUs),它们涉及变形菌门(α-,β-,γ-)、厚壁菌门和绿湾菌门3个细菌门类,其中,α-变形菌有3个OTUs,分别属于Altererythrobacter,Chelativorans和Sphingomonas菌属;β-变形菌有3个OTUs,它们属于Hydrogenophaga和Thiobacillus菌属;γ-变形菌有4个OTUs,它们属于Acinetobacter和Pseudomonas菌属;厚壁菌门有2个OTUs,分别属于Faecalibacterium和Bacillus菌属;绿湾菌门有暂时无法确定菌属的1个OTU。

 

关键字: 油页岩矿;细菌多样性;PCR-DGGE;16S rDNA;茂名

Composition of bacteria in soil from oil shale mine inMaoming using PCR-DGGE technique
JIANG Shaoyan1, WANG Wenxing2, XUE Xiangxin1, XU Kun2

1. School of Materials and Metallurgy, Northeastern University, Shenyang 110819, China;
2. College of Life and Health Sciences, Northeastern University, Shenyang 110819, China

Abstract:Improved SDS-high-salt extraction method was used to extract microbial total DNA from the soil of multi-point collection. PCR-DGGE was applied to analyze the community structure of bacteria in soil from oil shale mine in Maoming of Guangdong Province. The results show that a total of thirteen operational taxonomic units (OTUs) are isolated from the PCR-DGGE patterns. Proteobacteria (α-, β-, γ-), Firmicutes and Chloroflexi are the dominant bacterial communities in this habitat. Ten OTUs which belong to seven genera of Proteobacteria including Altererythrobacter, Chelativorans, Sphingomonas, Hydrogenophaga, Thiobacillus, Acinetobacter and Pseudomonas are identified. Two OTUs which belong to two genera of Firmicutesincluding Faecalibacterium and Bacillus are identified, and one OTU which belongs to unidentified genus of Chloroflexi is also identified.

 

Key words: oil shale mine; bacterial diversity; PCR-DGGE; 16S rDNA; Maoming

中南大学学报(自然科学版)
  ISSN 1672-7207
CN 43-1426/N
ZDXZAC
中南大学学报(英文版)
  ISSN 2095-2899
CN 43-1516/TB
JCSTFT
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