自然科学版 英文版
自然科学版 英文版
自然科学版 英文版

您目前所在的位置:首页 - 期刊简介 - 详细页面

中南大学学报(自然科学版)

Journal of Central South University

第48卷    第3期    总第271期    2017年3月

[PDF全文下载]    [Flash在线阅读]

    

文章编号:1672-7207(2017)03-0625-10
掺Mg与接枝RGD的HAnps载体系统对MG63细胞胞吞功能的影响
陈恬1, 2,陈良建1, 3,贺惠莉1,彭静2,郭筱慧2,郑雅亦1,邵春生1

(1. 中南大学 湘雅三医院,湖南 长沙,410013;
2. 中南大学 湘雅医学院,湖南 长沙,410013;
3. 中南大学 粉末冶金国家重点实验室,湖南 长沙,410083
)

摘 要: 采用水热合成法制备HAnps和掺镁HAnps (Mg-HAnps),通过硅烷化联合碳二亚胺法处理HAnps和Mg-HAnps后接枝黏附肽(RGD),以香豆素6(coumarin-6)为荧光探针标记HAnps,Mg-HAnps,RGD-HAnps和RGD-Mg-HAnps,并将其与人成骨肉瘤MG63细胞株(MG63)细胞共培养,研究掺Mg与接枝RGD的HAnps对MG63细胞胞吞作用的影响。研究结果表明:RGD-Mg-HAnps无细胞毒性,掺Mg与接枝RGD有利于MG63细胞胞吞HAnps颗粒,并有协同作用,RGD-Mg-HAnps具有介导胞吞的作用。

 

关键字: 基因载体;掺Mg羟基磷灰石纳米颗粒(Mg-HAnps);黏附肽(RGD);靶向性;胞吞作用

Influence of HAnps carrier system with doping Mg and grafting RGD on endocytosis of MG63 cells
CHEN Tian1, 2, CHEN Liangjian1, 3, HE Huili1, PENG Jing2, GUO Xiaohui2, ZHENG Yayi1, SHAO Chunsheng1

1. Third Xiangya Hospital, Central South University, Changsha 410013, China;
2. Xiangya School of Medicine, Central South University, Changsha 410013, China;
3. State Key Laboratory of Powder Metallurgy, Central South University, Changsha 410083, China

Abstract:HAnps and HAnps with doped Mg (Mg-HAnps) were prepared by hydrothermal synthesis method (HTSM), grafted adhesive peptide (RGD) after silanization combined with carbodiimide method, labeled HAnps, Mg-HAnps, RGD-HAnps, RGD-Mg-HAnps with coumarin-6 as a fluorescent label, and they were co-cultured with human osteosarcoma MG63 cell line (MG63) cells. The effects of HAnps with doping Mg and grafting RGD on endocytosis of MG63 cells were investigated. The results show that RGD-Mg-HAnps have no cytotoxicity. Mg-doped and RGD-grafted HAnps improve endocytosis of MG63 cells, which have synergistic effects. RGD-Mg-HAnps are able to promote endocytosis.

 

Key words: gene vector; Mg-doped Hydroxyapatite nanoparticles (Mg-HAnps); arginine-glycine-aspartic acid (RGD); targeting specificity; endocytosis

中南大学学报(自然科学版)
  ISSN 1672-7207
CN 43-1426/N
ZDXZAC
中南大学学报(英文版)
  ISSN 2095-2899
CN 43-1516/TB
JCSTFT
版权所有:《中南大学学报(自然科学版、英文版)》编辑部
地 址:湖南省长沙市中南大学 邮编: 410083
电 话: 0731-88879765(中) 88836963(英) 传真: 0731-88877727
电子邮箱:zngdxb@csu.edu.cn 湘ICP备09001153号