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中南大学学报(英文版)

Journal of Central South University

Vol. 27    No. 5    May 2020

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Changes of microbial diversity during pyrite bioleaching
YIN Lu(殷璐), YANG Hong-ying(杨洪英), LI Xiang(李想), TONG Lin-lin(佟琳琳), JIN Zhe-nan(金哲男), ZHANG Qin(张勤)

School of Metallurgy, Northeastern University, Shenyang 110819, China

Abstract:Microorganisms, one of the key factors affecting the bioleaching process, change the components of extracellular polymeric substance (EPS) and community structure to survive in leaching environments. In this work, Fourier transform infrared (FTIR), X-ray powder diffraction (XRD) and 16S rDNA high-throughput sequence analyses were used to reveal the microbial changes in planktonic and sessile phases during bioleaching. The results showed the occupation of sessile cells decreased from 66.2% to (10±3)%. After bioleaching, the planktonic and sessile cells have similar EPS, but they are different from the original cells. Pyrite dissolution mainly occurs at the early and late stages with the decreasing of particle diameter, by 50% and 40%, respectively. The 16S rDNA gene based sequence analysis results in total of 1117420 Reads across the six samples, presented among 7 phyla, 9 classes, 17 orders, 23 families and 31 genera. Genera Leptospirillum and Sulfobacillus are the main bacteria at the early and middle stages, and Leptospirillum is the main genus at the end of bioleaching. Aquabacterium and Acidovorax are special genera in sessile cells and Weissella is special in planktonic ones.

 

Key words: pyrite dissolution; sessile cells; planktonic cells; high-throughput sequence analysis; microbial diversity; bioleaching stage

中南大学学报(自然科学版)
  ISSN 1672-7207
CN 43-1426/N
ZDXZAC
中南大学学报(英文版)
  ISSN 2095-2899
CN 43-1516/TB
JCSTFT
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