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中南大学学报(自然科学版)

Journal of Central South University

第41卷    第4期    总第194期    2010年8月

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文章编号:1672-7207(2010)04-1628-05
愈创木酚与复合碳源共降解对黄孢原毛平革菌产酶的影响
冯冲凌,曾光明,黄丹莲,胡霜,苏峰峰,赵美花,赖萃

(湖南大学 环境科学与工程学院,湖南 长沙,410082)

摘 要: 通过正交实验法,研究愈创木酚与复合碳源共降解对黄孢原毛平革菌产酶的影响,同时探讨不同分子结构对酶催化机制的影响。实验结果表明:同时添加愈创木酚及不同结构的碳源对该菌产酶有显著影响,高浓度的愈创木酚对产酶有明显的促进作用;在培养基中添加愈创木酚2 mmol/L、葡萄糖 2.5 g/L和糊精 5 g/L,可以显著提高黄孢原毛平革菌的综合产酶能力;黄孢原毛平革菌可以分泌纤维素酶和木聚糖酶,而且二者之间有较好的线性正相关关系。

 

关键字: 愈创木酚;复合碳源;黄孢原毛平革菌

Influence of guaiacol and compounded carbon sources on enzymes of Phanerochaete chrysosporium
FENG Chong-ling, ZENG Guang-ming, HUANG Dan-lian, HU Shuang,
 SU Feng-feng, ZHAO Mei-hua, LAI Cui

College of Environmental Science and Engineering, Hunan University, Changsha 410082, China

Abstract:The influence of guaiacol and compounded carbons sources co-degradation on enzymes of white-rot fungi was studied through the orthogonal experiment. The results show that carbons with different structures and guaiacol have remarkable effects on enzymes secreting by Phanerochaete chrysosporium. High concentration of guaiacol can enhance enzymes production. After optimumization of various factors, the addition of guaiacol 2 mmol/L, glucose 2.5 g/L, dextrine 5 g/L in culture medium can significantly promote enzymes production. CMCase and xylanase produced by Phanerochaete chrysosporium are little affected by exterior environment. According to the correlativity between the CMCase and the xylanase analyzed using linear regression, a positive correlation the CMCase and the xylanase is found.

 

Key words: guaiacol; compounded carbons; Phanerochaete chrysosporium

中南大学学报(自然科学版)
  ISSN 1672-7207
CN 43-1426/N
ZDXZAC
中南大学学报(英文版)
  ISSN 2095-2899
CN 43-1516/TB
JCSTFT
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