自然科学版 英文版
自然科学版 英文版
自然科学版 英文版

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中南大学学报(英文版)

Journal of Central South University

Vol. 16    No. 2    April 2009

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Bioleaching of chalcocite by mixed microorganisms subjected to mutation
KANG Jian(康 健)1, 2, QIU Guan-zhou(邱冠周)1, GAO Jian(高 健)2,
WANG Hai-hua (王海华) 2, WU Xue-ling(吴学玲)1, DING Jian-nan(丁建南)1<

1.School of Resources Processing and Bioengineering, Central South University,
Changsha 410083, China;
2. School of Life Science, Hunan University of Science and Technology, Xiangtan 411201, China

Abstract: Mixed microorganisms with elevated activity of chalcocite-leaching were screened by mutation methods. The original microorganisms collected from acid mine drainage of different sites were mixed and then treated with mutagens NO2, diethyl sulfate (DES), UV and their combinations, respectively. Five groups of mixed microorganisms with much stronger ore-leaching ability were obtained by screening on the leaching media. Among them, group E of mixed microorganisms (treated with 1% DES for 60 min) with the best performance on chalcocite-leaching, increases the content of Cu2+ by 101.4% in 20 d of leaching compared with the control culture. In addition, group E is more tolerant to Cu2+ in media than the control without mutation treatment. Analysis for the diversity of microbial clones indicates that half of operational taxonomic units (OTUs) in group E are Acidithiobacillus ferrooxidans. These observations suggest that group E might have potentials for industrial application.

 

Key words: chalcocite; mixed microorganisms; bioleaching; mutation

中南大学学报(自然科学版)
  ISSN 1672-7207
CN 43-1426/N
ZDXZAC
中南大学学报(英文版)
  ISSN 2095-2899
CN 43-1516/TB
JCSTFT
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