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中南大学学报(英文版)

Journal of Central South University

Vol. 16    No. 2    April 2009

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A novel salt-tolerant Micrococcus sp. DUT_AHX capable of degrading nitrobenzene
AI Hai-xin(艾海新)1, 2, ZHOU Ji-ti(周集体)2, LÜ Hong(吕 红)2

1. School of Life Science, Liaoning University, Shenyang 110036, China;
2. School of Environment and Biological Science and Technology, Dalian University of Technology,
Dalian 116024, China

Abstract: A novel strain of Micrococcus sp. DUT_AHX, which was isolated from the sludge of a nitrobenzene (NB)-manufacturing plant and could utilize NB as the sole carbon source, was identified on the basis of physiological and biochemical tests and 16S ribosomal DNA (rDNA) sequence analysis. It can grow at the temperature up to 40 ℃ or in the presence of NaCl concentration up to 12 g/L in Luria-Bertani (LB) medium. The optimal degradation conditions are as follows: temperature 37 ℃, pH 7.0, and shaking speed 150 r/min. The strain involves a partial reductive pathway due to the release of ammonia and can also utilize 2-aminophenol as the sole carbon source. Furthermore, the enzyme activity tests show that crude extracts of NB-grown strain DUT_AHX mainly contain 2-aminophenol 1, 6-dioxygenase activity. The exploitation of salt-tolerant bacteria will be a remarkable improvement in NB bioremediation and wastewater treatment at high salinity and high temperature.

 

Key words: Micrococcus; 16S rDNA; nitrobenzene; degradation; salt-tolerant

中南大学学报(自然科学版)
  ISSN 1672-7207
CN 43-1426/N
ZDXZAC
中南大学学报(英文版)
  ISSN 2095-2899
CN 43-1516/TB
JCSTFT
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