自然科学版 英文版
自然科学版 英文版
自然科学版 英文版

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中南大学学报(英文版)

Journal of Central South University

Vol. 13    No. 2    April 2006

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Extraction optimization of coumarins from radix angelicae pubescentis
by HPLC-DAD coupled with uniform design
GUO Fang-qiu(郭方遒) 1, HUANG Lan-fang(黄兰芳)1,2, ZHOU Huo-fei(周火飞) 1,
JIANG Qiu-ju(江秋菊)1

1. School of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China;
2.Technical Center of Changde Cigarette Factory, Changde 415000, China

Abstract:Uniform design was used to optimize extraction condition of direct refluence extraction of coumarins from the Chinese traditional medicine of radix angelicae pubescentis(Duhuo); the sum peak area of coumarins separated with high performance liquid chromatography(HPLC) at detection wavelength of 320 nm was considered as detection index, two factors of solvent concentration and extraction time were mainly studied at extraction temperature of 85℃and a volume ratio of solvent to sample of 10∶1. Optimal subclass, quadric polynomial step by step aggression and neural network method were applied to process the experimental results. The results show that the first and second methods give the same factors combination (concentration of ethanol: 95%, extraction time: 3.6 h) and the second method is much better than the first one. The extraction model is consequently developed.

 

Key words: radix angelicae pubescentis(Duhuo); couamrins; uniform design; high performance liquid chromata graphy-diode array detector(HPLC-DAD)

中南大学学报(自然科学版)
  ISSN 1672-7207
CN 43-1426/N
ZDXZAC
中南大学学报(英文版)
  ISSN 2095-2899
CN 43-1516/TB
JCSTFT
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