自然科学版 英文版
自然科学版 英文版
自然科学版 英文版

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中南大学学报(英文版)

Journal of Central South University

Vol. 8    No. 3    September 2001

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Biocompatibility evaluation in vitro. Part III: Cytotoxicity
expression of human and animal osteoblasts on the biomaterials
RUAN Jian-ming1,M.H. Grant2

1. State Key Laboratory for PowderMetallurgy, Central SouthUniversity, Changsha 410083, China;
2. BioengineeringUnit, Strathclyde University, Glasgow, G4 0NW, UK

Abstract:Exclusion and inflammation in the clinic are observed for various reasons including material chemical composition, physical properties aswell as macro-and micro-structure of the implants, surface condition of the implants, and also patient dependent factors. Cytotoxicity expression of cells is a central issue in current biocompatibility to screen the potential implantmaterials and drugs. This studywas aimed at investigation reaction between the potential implantmaterials and surround tissue. Cytotoxicityof human and ratosteoblast in the material extracts was determinated by testing standards such as GHS assay, MTT assay, alkaline phosphatase activity assay, LDH assay, and Lowry assay. Research results demonstrated that compared with the control condition polystyrene culture plate both human and rat osteoblast cells have normal phenotypic expression in hydroxyapatite extract, and this expression was statistically restricted in hydroxyapatite-spinel extract. However, this restrict, e.g. cytotoxicity could be partially eliminated by immersion treatment of the materials in culture medium.

 

Key words: biomaterial; cytotoxicity; biological molecular technique

中南大学学报(自然科学版)
  ISSN 1672-7207
CN 43-1426/N
ZDXZAC
中南大学学报(英文版)
  ISSN 2095-2899
CN 43-1516/TB
JCSTFT
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