自然科学版 英文版
自然科学版 英文版
自然科学版 英文版

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中南大学学报(英文版)

Journal of Central South University

Vol. 17    No. 1    February 2010

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Isolation and characterization of acidophilic bacterium from Dongxiangshan Mine in Xinjiang Province, China
XIA Jin-lan(夏金兰)1, 2, ZHANG Qian(张倩)1, 2, ZHANG Rui-yong(张瑞永)1, 2, PENG Juan-hua(彭娟花)1, 2,
PENG An-an(彭安安)1, 2, ZHAO Xiao-ju

1. Key Laboratory of Biometallurgy of Ministry of Education, Central South University, Changsha 410083, China;
2. School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China

Abstract:One bioleaching bacterium, named as strain DXS, was isolated from acid mine drainages (AMDs) of Dongxiangshan Mine of Hami, Xinjiang Province, China. The strain DXS is gram-negative and rod-shaped with a size of (0.40±0.05) μm×(1.3±0.5) μm. The optimal temperature and pH for growth are 30 ℃ and pH 2.0, respectively. It can grow autotrophically by using ferrous iron, elemental sulfur and NaS2O3 as sole energy sources. In the phylogenetic tree, strain DXS has similarity with Acidithiobacillus ferrooxidans type strain ATCC 23270 with 99.57% sequence similarity. The cloning and sequencing of Iro protein gene (iro) and tetrathionate hydrolase gene (tth) reveal that strain DXS is completely identical in iro gene sequence to A. ferrooxidans LY (DQ166841), and almost identical in tth gene sequene to A. ferrooxidans (AB259312) (only two nucleotides change). The bioleaching experiments of marmatite and pyrite reveal that the leached zinc and iron concentrations reach 3.01 g/L and 2.75 g/L, respectively. The strain has a well potential application in industry bioleaching.

 

Key words: Acidithiobacillus ferrooxidans; bioleaching; iro and tth gene; 16S rRNA

中南大学学报(自然科学版)
  ISSN 1672-7207
CN 43-1426/N
ZDXZAC
中南大学学报(英文版)
  ISSN 2095-2899
CN 43-1516/TB
JCSTFT
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